Determination of Orotic Acid, Uric Acid, and Creatinine in Milk by Liquid Chromatography
نویسندگان
چکیده
منابع مشابه
Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography.
An environmentally friendly reversed-phase HPLC method for simultaneous determination of creatinine and uric acid in human urine samples has been developed. Human urine samples were pretreated by dilution, protein precipitation, centrifugation and filtration, followed by HPLC separations using a reversed-phase C(18) column with an aqueous mobile phase of phosphate buffer. The retention loss of ...
متن کاملDetermination of Orotic acid in milk
Method VFD0134J_E HPLC Column: Eurokat H, 10 μm, 300 x 8 mm Order No. 30GX340EKN Phase: Eurokat H, 10 μm Eluent: 0.01 N Sulfuric acid Gradient: Isocratic Flow rate: 0.6 ml/min Temperature: 60 °C Volume: 20 μL (Standard) Detection: UV, 210 nm Substances: Orotic acid, Lactic acid and Acetic acid
متن کاملOrotic acid in milk.
Other studies in this laboratory (1) drew our attention to the possibility that erotic acid might be concerned with the maintenance of adequate lactation in the rat and with the survival of the young. Since no data were available on the erotic acid content of milk, this study was undertaken. The occurrence of erotic acid as a component of milk has been indicated since 1904, when Biscaro and Bel...
متن کاملMeasurement of serum uric acid by liquid chromatography.
The biochemical and clinical importance of uric acid in gout, the Lesch-Nyhan syndrome, and several other disease states has recently been discussed (1). Serum uric acid is now commonly measured by spectrophotometry. The spectrophotometric methods, which account for more than 99% of the assays put to routine use, can be subdivided into two categories: redox colorimetric and enzymatic (uricase) ...
متن کاملQuantitation of urinary uric acid by reversed-phase liquid chromatography.
The proposed isocratic liquid-chromatographic method for uric acid has good performance characteristics. Results by this procedure and by an enzymic method for uric acid correlated well (r = 0.986). Our method is inexpensive, rapid, and considerably simpler than other techniques for determining uric acid in urine.
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2004
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/87.1.116